Informatics Publishing Limited

M. Shankar ¹, N. L. Gowrishankar ¹, S. Narendiran ¹, K. Sudhakar ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Swami Vivekanandha Institute of Pharmaceutical Sciences, Vangapally (V), Yadhagirigutta (M), Nalgonda (District), Pin - 508286, Andhra Pradesh, IN

Abstract

The protein-ligand interaction plays a significant role in the structural based drug designing. In our research work we have taken the cyclo-oxygenase receptor (COX). The receptor was docked to the commercially available NSAID (Non Steroid Anti Inflammatory Drugs) Celecoxib and Naproxen Protein Structure Modeling Method, using INSIGHTII software. We tried to improve the binding efficiency and steric compatibility of Celecoxib and Naproxen against COX receptor. Several modifications were made to the probable functional groups which were interacting with receptor molecule. Analogs of the drug molecule were prepared using MODELLER9v1chemsketch and docked using INSIGHT II docking software. The modified drugs was sketched using chemsketch were found to be better than the convectional drugs available. Further from this work we can improve the steric compatibility and then Absorption, Distribution, Metabolism and Excretion (ADME) properties of the analogs can be analyzed using Insilco ADME tools.

Keywords

Insight II, Modeller 9V1, Savs, Rampage, Patchdock, Rasmol.

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T. Venkatachalam ¹, S. Narendiran ¹, P. Kalai Selvi ¹, P. Dheen Kumar ²

Affiliations
1 Dept. of Pharmaceutical Chemistry, JKKMMRF College of Pharmacy, Komara Palayam-638183, Tamil Nadu, IN
2 Dept. of Pharmaceutical Chemistry, Maharaji College of Pharmacy, Chennai-600090, Tamil Nadu, IN

Abstract

A simple, precise, accurate and rapid HPLC method has been developed, and validated for the determination of ofloxacin and satranidazole simultaneously, in combined dosage form. Acetonitrile and ammonium di-hydrogen orthophosphate buffer with ph 7.0 (50%:50%)is used as the mobile phase, 300 is the detection wavelength for this study. The applicability of the method for simultaneous determinations of oflaxacin and satranidazole was verified by the determination of these compounds in marketed tablets .Results of the analysis were validated statistically, and by recovery studies (99.43-101.28%). The recovery and RSD values with in limits given in ICH guidelines method developments indicates that the suitability of proposed for the routine determination of the these compounds in tablets .the validation parameters; linearity (r=9999) sensitivity (LOD (1.0-1.5×10^-4), LOQ (3.0-4.5×10^-4) accuracy (99.43-101.28%) and reproducibility were found to satisfactory. The proposed method can be successfully used to determine the drug contents of marketed formulation.

Keywords

RP-HPLC, Oflaxacin, Satranidazole, Validation.

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